The IHPRF1 (Infantile, Hypotonia, with Psychomotor Retardation and Characteristic Facies 1) and CLIFAHDD (Congenital contractures of the Limbs and FAce, Hypotonia, and Developmental Delay) syndromes are two severe neurodevelopmental disorders linked to mutations in the NALCN gene. The sodium (Na+) leak channel NALCN is a crucial regulator of
cell excitability by setting up the resting membrane potential of neurons and endocrine cells (Cochet-Bissuel et al, 2014; Guérineau et al, 2021). The IHPRF1 pathogenic variants are inherited autosomal recessive loss-of-function while CLIFAHDD variants are dominant, de novo gain-of-function (Chong et al, 2015; Bouasse et al, 2019). Both syndromes display an
onset early in infancy and may lead to premature death. Patients exhibit a large panel of symptoms of variable severity, and there is yet no available treatment. Over the last years, we have developed a wide range of tools (from cell to animal models) to study the role of NALCN in both physiological and pathological conditions. Recently, we have generated animal models that carry NALCN mutations to characterize the corresponding developmental disorders. The first objective of the project will be to characterize the physiological defects in these models.
This will first be performed by using a combination of techniques including, but not restricted to, electrophysiological recordings (EEG, patch-clamp), calcium imaging, hormone release measurement, and at the behavioral levels using motor/cognitive tests. In a second step, gene therapy approaches will be assessed in those animal models to make the proof of principle that they represent relevant therapeutic strategies to restore normal phenotypes.
- PhD or MD/PhD with a background in Physiology and/or Neuroscience
- Excellent research records and academic activities
- Strong expertise in wet-lab experience with advanced techniques (i.e., Patch-clamp,
Viral-mediated gene transfer in animal models, Calcium imaging) and quantitative data
- Experience in mouse behavior assessment and analysis is a pre-requisite. Holding an
EU accreditation to conduct experiments in animals will be a plus.
- Be imaginative, open, dynamic, team-worker, strongly self-driven and well organized
How to apply?
Applicants should send an e-mail to Dr. Arnaud Monteil (firstname.lastname@example.org) with a CV including a list of publications and technical expertise, a description of research interests and at least two names of individuals willing to act as references for the candidate.
Application review will begin immediately and will continue until a suitable candidate is selected. The position will preferentially start July 1st, 2022